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Mem 117 c19-dna barcoding africa’s endemic cycads. sold by nybgshop.org for $4.99

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This purchase only includes chapter 19 of this title.

Abstract

Africa√Ę‚ā¨‚ĄĘs cycads (68 taxa in two endemic genera, Encephalartos and Stangeria) are endangered,¬†with four species extinct in the wild and 35% threatened (EN, CR or EW). Although¬†legislation is strict, threats from illegal collection for horticulture and medicine are rife.¬†Often seizures are unidentifiable, while current legislation demands identification for permits.¬†Ex situ conservation of genetic¬†and locality-based¬†diversity is paramount. Individuals¬†lacking geographic and diagnostic information are also difficult to identify. Species delimitation¬†and numbers are uncertain. DNA barcoding will¬†assist with all the above-mentioned¬†issues. Here,¬†proposed (matK and rbcLa) and additional (psbA-trnH¬†and nrITS1) DNA¬†barcodes were¬†tested on ~350 samples representing all but one taxon. Results show good¬†amplification and sequencing success of proposed barcoding regions. Genetic¬†variation,¬†however, is extremely low, as is resolution at a specific level, even when multilocus barcodes¬†are employed. A multitiered barcoding strategy is proposed and identifiable species listed.

Keywords: Barcoding, multi-tiered, low genetic variation, Cycadales, Zamiaceae, Encephalartos, Stangeria




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